Cenacle / The Last Supper Room is located on Mount Zion, south of Dormition Abbey, is the Cenacle or Coenaculum (‘upper room’) which is believed to be the site where Jesus gathered his disciples for the Last Supper. The site is also believed by some to be the Tomb of David.

The site has undergone many transformations throughout its history. The current two-floor structure was built by the Franciscans during the 14th century on the ruins of former Roman, Byzantine, and Crusader Churches. In 1524, the Church was transformed into a Mosque. A mihrab can still be found in a corner of the Church from this period. It was also during this time that the designation of this site as the burial place of the Prophet David (Nabi Daoud) was solidified, a belief which continues today. Under Ottoman rule, the site was returned to use as a Church.

Since 1948, the site has been under Israeli control and the lower floor, believed to house the Tomb of David, has been transformed into a synagogue as well as a memorial to victims of the Holocaust (Chamber of the Holocaust). The Cenacle, located on the upper floor, is open to visitors, but the Franciscans are only permitted to hold mass there twice a year, on the Day of Pentecost and on Holy Thursday.